A Genome-wide Haploid Genetic Screen Identifies Regulators of Glutathione Abundance and Ferroptosis Sensitivity

Jennifer Yinuo Cao, Aunoy Poddar, Leslie Magtanong, Jennifer H. Lumb, Trevor R. Mileur, Michael A. Reid, Cole M. Dovey, Jin Wang, Jason W. Locasale, Everett Stone, Susan P.C. Cole, Jan E. Carette, Scott J. Dixon

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Glutathione suppresses the non-apoptotic cell death process of ferroptosis. Using genome-wide human haploid cell mutagenesis and FACS-based detection, Cao et al. identify negative regulators of intracellular glutathione abundance that affect glutathione efflux and NRF2 protein levels, altering ferroptosis sensitivity.

Original languageEnglish (US)
Pages (from-to)1544-1556.e8
JournalCell Reports
Volume26
Issue number6
DOIs
StatePublished - Feb 5 2019

Fingerprint

Haploidy
Glutathione
Genes
Genome
Mutagenesis
Human Genome
Cell death
Cell Death
Proteins

Keywords

  • cancer
  • collateral sensitivity
  • ferroptosis
  • glutathione
  • iron
  • metabolite efflux
  • multidrug resistance
  • necrosis
  • ROS

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Cao, J. Y., Poddar, A., Magtanong, L., Lumb, J. H., Mileur, T. R., Reid, M. A., ... Dixon, S. J. (2019). A Genome-wide Haploid Genetic Screen Identifies Regulators of Glutathione Abundance and Ferroptosis Sensitivity. Cell Reports, 26(6), 1544-1556.e8. https://doi.org/10.1016/j.celrep.2019.01.043

A Genome-wide Haploid Genetic Screen Identifies Regulators of Glutathione Abundance and Ferroptosis Sensitivity. / Cao, Jennifer Yinuo; Poddar, Aunoy; Magtanong, Leslie; Lumb, Jennifer H.; Mileur, Trevor R.; Reid, Michael A.; Dovey, Cole M.; Wang, Jin; Locasale, Jason W.; Stone, Everett; Cole, Susan P.C.; Carette, Jan E.; Dixon, Scott J.

In: Cell Reports, Vol. 26, No. 6, 05.02.2019, p. 1544-1556.e8.

Research output: Contribution to journalArticle

Cao, JY, Poddar, A, Magtanong, L, Lumb, JH, Mileur, TR, Reid, MA, Dovey, CM, Wang, J, Locasale, JW, Stone, E, Cole, SPC, Carette, JE & Dixon, SJ 2019, 'A Genome-wide Haploid Genetic Screen Identifies Regulators of Glutathione Abundance and Ferroptosis Sensitivity', Cell Reports, vol. 26, no. 6, pp. 1544-1556.e8. https://doi.org/10.1016/j.celrep.2019.01.043
Cao, Jennifer Yinuo ; Poddar, Aunoy ; Magtanong, Leslie ; Lumb, Jennifer H. ; Mileur, Trevor R. ; Reid, Michael A. ; Dovey, Cole M. ; Wang, Jin ; Locasale, Jason W. ; Stone, Everett ; Cole, Susan P.C. ; Carette, Jan E. ; Dixon, Scott J. / A Genome-wide Haploid Genetic Screen Identifies Regulators of Glutathione Abundance and Ferroptosis Sensitivity. In: Cell Reports. 2019 ; Vol. 26, No. 6. pp. 1544-1556.e8.
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