Arrestin-3 interaction with maternal embryonic leucine-zipper kinase

Nicole A. Perry, Kevin P. Fialkowski, Tamer S. Kaoud, Ali I. Kaya, Andrew L. Chen, Juliana M. Taliaferro, Vsevolod V. Gurevich, Kevin Dalby, T. M. Iverson

Research output: Contribution to journalArticle

Abstract

Maternal embryonic leucine-zipper kinase (MELK) overexpression impacts survival and proliferation of multiple cancer types, most notably glioblastomas and breast cancer. This makes MELK an attractive molecular target for cancer therapy. Yet the molecular mechanisms underlying the involvement of MELK in tumorigenic processes are unknown. MELK participates in numerous protein-protein interactions that affect cell cycle, proliferation, apoptosis, and embryonic development. Here we used both in vitro and in-cell assays to identify a direct interaction between MELK and arrestin-3. A part of this interaction involves the MELK kinase domain, and we further show that the interaction between the MELK kinase domain and arrestin-3 decreases the number of cells in S-phase, as compared to cells expressing the MELK kinase domain alone. Thus, we describe a new mechanism of regulation of MELK function, which may contribute to the control of cell fate.

Original languageEnglish (US)
Article number109366
JournalCellular Signalling
Volume63
DOIs
StatePublished - Nov 1 2019

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Leucine Zippers
Phosphotransferases
Mothers
beta-Arrestin 2
Glioblastoma
S Phase
Embryonic Development
Neoplasms
Cell Cycle
Proteins
Cell Count

Keywords

  • Arrestin
  • Cell fate signaling
  • Maternal embryonic leucine-zipper kinase (MELK)
  • Protein-protein interactions

ASJC Scopus subject areas

  • Cell Biology

Cite this

Perry, N. A., Fialkowski, K. P., Kaoud, T. S., Kaya, A. I., Chen, A. L., Taliaferro, J. M., ... Iverson, T. M. (2019). Arrestin-3 interaction with maternal embryonic leucine-zipper kinase. Cellular Signalling, 63, [109366]. https://doi.org/10.1016/j.cellsig.2019.109366

Arrestin-3 interaction with maternal embryonic leucine-zipper kinase. / Perry, Nicole A.; Fialkowski, Kevin P.; Kaoud, Tamer S.; Kaya, Ali I.; Chen, Andrew L.; Taliaferro, Juliana M.; Gurevich, Vsevolod V.; Dalby, Kevin; Iverson, T. M.

In: Cellular Signalling, Vol. 63, 109366, 01.11.2019.

Research output: Contribution to journalArticle

Perry, NA, Fialkowski, KP, Kaoud, TS, Kaya, AI, Chen, AL, Taliaferro, JM, Gurevich, VV, Dalby, K & Iverson, TM 2019, 'Arrestin-3 interaction with maternal embryonic leucine-zipper kinase', Cellular Signalling, vol. 63, 109366. https://doi.org/10.1016/j.cellsig.2019.109366
Perry NA, Fialkowski KP, Kaoud TS, Kaya AI, Chen AL, Taliaferro JM et al. Arrestin-3 interaction with maternal embryonic leucine-zipper kinase. Cellular Signalling. 2019 Nov 1;63. 109366. https://doi.org/10.1016/j.cellsig.2019.109366
Perry, Nicole A. ; Fialkowski, Kevin P. ; Kaoud, Tamer S. ; Kaya, Ali I. ; Chen, Andrew L. ; Taliaferro, Juliana M. ; Gurevich, Vsevolod V. ; Dalby, Kevin ; Iverson, T. M. / Arrestin-3 interaction with maternal embryonic leucine-zipper kinase. In: Cellular Signalling. 2019 ; Vol. 63.
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