Biophysical basis for noninvasive skin cancer detection using Raman spectroscopy

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

Raman spectroscopy (RS) is proving to be a valuable tool for real time noninvasive skin cancer detection via optical fiber probe. However, current methods utilizing RS for skin cancer diagnosis rely on statistically based algorithms to provide tissue classification and do not elucidate the underlying biophysical changes of skin tissue. Therefore, we aim to use RS to explore skin biochemical and structural characteristics and then correlate the Raman spectrum of skin tissue with its disease state. We have built a custom confocal micro-Raman spectrometer system with an 830nm laser light. The high resolution capability of the system allows us to measure spectroscopic features from individual tissue components in situ. Raman images were collected from human skin samples from Mohs surgical biopsy, which were then compared with confocal laser scanning, two-photon fluorescence and hematoxylin and eosin-stained images to develop a linear model of skin tissue Raman spectra. In this model, macroscopic tissue spectra obtained from RS fiber probe were fit into a linear combination of individual basis spectra of primary skin constituents. The fit coefficient of the model explains the biophysical changes spanning a range of normal and various disease states. The model allows for determining parameters similar to that a pathologist is familiar reading and will be a significant guidance in developing RS diagnostic decision schemes.

Original languageEnglish (US)
Title of host publicationBiomedical Vibrational Spectroscopy 2016
Subtitle of host publicationAdvances in Research and Industry
EditorsAnita Mahadevan-Jansen, Wolfgang Petrich
PublisherSPIE
ISBN (Electronic)9781628419382
DOIs
StatePublished - Jan 1 2016
EventBiomedical Vibrational Spectroscopy 2016: Advances in Research and Industry - San Francisco, United States
Duration: Feb 13 2016Feb 14 2016

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9704
ISSN (Print)1605-7422

Other

OtherBiomedical Vibrational Spectroscopy 2016: Advances in Research and Industry
CountryUnited States
CitySan Francisco
Period2/13/162/14/16

Fingerprint

Raman Spectrum Analysis
Skin Neoplasms
Raman spectroscopy
Skin
cancer
Tissue
Lasers
Raman spectra
Optical Fibers
Raman scattering
probes
Hematoxylin
Eosine Yellowish-(YS)
Photons
lasers
Biopsy
Reading
Linear Models
Reference Values
optical fibers

Keywords

  • Raman spectroscopy
  • biophysical model
  • noninvasive detection
  • skin cancer
  • spectral diagnosis

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Feng, X., Moy, A. J., Markey, M. K., Fox, M. C., Reichenberg, J. S., & Tunnell, J. W. (2016). Biophysical basis for noninvasive skin cancer detection using Raman spectroscopy. In A. Mahadevan-Jansen, & W. Petrich (Eds.), Biomedical Vibrational Spectroscopy 2016: Advances in Research and Industry [97040C] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9704). SPIE. https://doi.org/10.1117/12.2209421

Biophysical basis for noninvasive skin cancer detection using Raman spectroscopy. / Feng, Xu; Moy, Austin J.; Markey, Mia K.; Fox, Matthew C.; Reichenberg, Jason S.; Tunnell, James W.

Biomedical Vibrational Spectroscopy 2016: Advances in Research and Industry. ed. / Anita Mahadevan-Jansen; Wolfgang Petrich. SPIE, 2016. 97040C (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9704).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Feng, X, Moy, AJ, Markey, MK, Fox, MC, Reichenberg, JS & Tunnell, JW 2016, Biophysical basis for noninvasive skin cancer detection using Raman spectroscopy. in A Mahadevan-Jansen & W Petrich (eds), Biomedical Vibrational Spectroscopy 2016: Advances in Research and Industry., 97040C, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 9704, SPIE, Biomedical Vibrational Spectroscopy 2016: Advances in Research and Industry, San Francisco, United States, 2/13/16. https://doi.org/10.1117/12.2209421
Feng X, Moy AJ, Markey MK, Fox MC, Reichenberg JS, Tunnell JW. Biophysical basis for noninvasive skin cancer detection using Raman spectroscopy. In Mahadevan-Jansen A, Petrich W, editors, Biomedical Vibrational Spectroscopy 2016: Advances in Research and Industry. SPIE. 2016. 97040C. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.2209421
Feng, Xu ; Moy, Austin J. ; Markey, Mia K. ; Fox, Matthew C. ; Reichenberg, Jason S. ; Tunnell, James W. / Biophysical basis for noninvasive skin cancer detection using Raman spectroscopy. Biomedical Vibrational Spectroscopy 2016: Advances in Research and Industry. editor / Anita Mahadevan-Jansen ; Wolfgang Petrich. SPIE, 2016. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
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