DESI Spray Stability in the Negative Ion Mode Is Dependent on Relative Humidity

Clara L. Feider, Rachel J. DeHoog, Marta Sans, Jialing Zhang, Anna Krieger, Livia S. Eberlin

Research output: Contribution to journalArticle

Abstract

Ambient ionization mass spectrometry (MS) techniques, such as desorption electrospray ionization (DESI), have been increasingly used due to their simplicity, minimal sample preparation requirements, and potential applications in the field and the clinic. However, due to their intrinsic nature, the performance of these methods is susceptible to variations in ambient conditions. Here, we present data that suggests DESI-MS analysis becomes inconsistent below a relative humidity (RH) level of ~ 35%. At low RH, we hypothesize that the DESI spray is subjected to frequent electrical discharges, resulting in unstable ionization and atypical mass spectra. Consequentially, poor image quality is observed when used for tissue imaging. Our results suggest that RH control should be considered in DESI-MS experiments to assure data quality.

Original languageEnglish (US)
Pages (from-to)376-380
Number of pages5
JournalJournal of the American Society for Mass Spectrometry
Volume30
Issue number2
DOIs
StatePublished - Feb 15 2019

Fingerprint

Electrospray ionization
Humidity
Desorption
Atmospheric humidity
Negative ions
Electrospray Ionization Mass Spectrometry
Ions
Mass spectrometry
Ionization
Humidity control
Mass Spectrometry
Image quality
Tissue
Imaging techniques
Experiments

Keywords

  • Data quality
  • Desorption electrospray ionization
  • Humidity
  • Mass spectrometry imaging

ASJC Scopus subject areas

  • Structural Biology
  • Spectroscopy

Cite this

DESI Spray Stability in the Negative Ion Mode Is Dependent on Relative Humidity. / Feider, Clara L.; DeHoog, Rachel J.; Sans, Marta; Zhang, Jialing; Krieger, Anna; Eberlin, Livia S.

In: Journal of the American Society for Mass Spectrometry, Vol. 30, No. 2, 15.02.2019, p. 376-380.

Research output: Contribution to journalArticle

Feider, Clara L. ; DeHoog, Rachel J. ; Sans, Marta ; Zhang, Jialing ; Krieger, Anna ; Eberlin, Livia S. / DESI Spray Stability in the Negative Ion Mode Is Dependent on Relative Humidity. In: Journal of the American Society for Mass Spectrometry. 2019 ; Vol. 30, No. 2. pp. 376-380.
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