Design and Characterization of Nucleopeptides for Hydrogel Self-Assembly

Kiheon Baek, Alexander D. Noblett, Pengyu Ren, Laura J. Suggs

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Self-assembling peptides can be used in a bottom-up approach to build hydrogels that are similar to the extracellular matrix at both structural and functional levels. In this study, a nucleo-tripeptide library was constructed to identify molecules that form hydrogels under physiological conditions. We used both experimental and computational approaches to study these self-assembled structures. Circular dichroism spectroscopy, transmission electron microscopy, and rheometry were utilized to support and supplement molecular dynamics simulations. Our data demonstrate that nucleo-tripeptides can form nanofibrous hydrogels through Watson-Crick base pairing and π-πstacking interactions. Self-assembly conditions are mediated by nucleo-tripeptide hydrophobicity and amphiphilicity and can therefore be regulated by a rational molecular design. We have found that structures derived from specific peptide and nucleobase conjugations form hydrogels under physiologic conditions, making them promising candidates for biomedical applications.

Original languageEnglish (US)
Pages (from-to)2812-2821
Number of pages10
JournalACS Applied Bio Materials
Volume2
Issue number7
DOIs
StatePublished - Jul 15 2019

Fingerprint

Hydrogels
Hydrogel
Self assembly
Peptides
Circular dichroism spectroscopy
Molecular Dynamics Simulation
Hydrophobicity
Circular Dichroism
Transmission Electron Microscopy
Hydrophobic and Hydrophilic Interactions
Base Pairing
Libraries
Extracellular Matrix
Molecular dynamics
Spectrum Analysis
Transmission electron microscopy
Molecules
Computer simulation

Keywords

  • biocompatibility
  • hydrogel
  • molecular dynamics simulation
  • nanofiber
  • nucleopeptides
  • self-assembly
  • supramolecular chemistry

ASJC Scopus subject areas

  • Biomaterials
  • Chemistry(all)
  • Biomedical Engineering
  • Biochemistry, medical

Cite this

Design and Characterization of Nucleopeptides for Hydrogel Self-Assembly. / Baek, Kiheon; Noblett, Alexander D.; Ren, Pengyu; Suggs, Laura J.

In: ACS Applied Bio Materials, Vol. 2, No. 7, 15.07.2019, p. 2812-2821.

Research output: Contribution to journalArticle

Baek, Kiheon ; Noblett, Alexander D. ; Ren, Pengyu ; Suggs, Laura J. / Design and Characterization of Nucleopeptides for Hydrogel Self-Assembly. In: ACS Applied Bio Materials. 2019 ; Vol. 2, No. 7. pp. 2812-2821.
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