DNA fragility in the parallel evolution of pelvic reduction in stickleback fish

Kathleen T. Xie, Guliang Wang, Abbey C. Thompson, Julia I. Wucherpfennig, Thomas E. Reimchen, Andrew D.C. MacColl, Dolph Schluter, Michael A. Bell, Karen M. Vasquez, David M. Kingsley

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Evolution generates a remarkable breadth of living forms, but many traits evolve repeatedly, by mechanisms that are still poorly understood. A classic example of repeated evolution is the loss of pelvic hindfins in stickleback fish (Gasterosteus aculeatus). Repeated pelvic loss maps to recurrent deletions of a pelvic enhancer of the Pitx1 gene. Here, we identify molecular features contributing to these recurrent deletions. Pitx1 enhancer sequences form alternative DNA structures in vitro and increase double-strand breaks and deletions in vivo. Enhancer mutability depends on DNA replication direction and is caused by TG-dinucleotide repeats. Modeling shows that elevated mutation rates can influence evolution under demographic conditions relevant for sticklebacks and humans. DNA fragility may thus help explain why the same loci are often used repeatedly during parallel adaptive evolution.

Original languageEnglish (US)
Pages (from-to)81-84
Number of pages4
JournalScience
Volume363
Issue number6422
DOIs
StatePublished - Jan 4 2019

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Smegmamorpha
Fishes
Dinucleotide Repeats
DNA
Mutation Rate
DNA Replication
Demography
Genes
Direction compound
In Vitro Techniques

ASJC Scopus subject areas

  • General

Cite this

Xie, K. T., Wang, G., Thompson, A. C., Wucherpfennig, J. I., Reimchen, T. E., MacColl, A. D. C., ... Kingsley, D. M. (2019). DNA fragility in the parallel evolution of pelvic reduction in stickleback fish. Science, 363(6422), 81-84. https://doi.org/10.1126/science.aan1425

DNA fragility in the parallel evolution of pelvic reduction in stickleback fish. / Xie, Kathleen T.; Wang, Guliang; Thompson, Abbey C.; Wucherpfennig, Julia I.; Reimchen, Thomas E.; MacColl, Andrew D.C.; Schluter, Dolph; Bell, Michael A.; Vasquez, Karen M.; Kingsley, David M.

In: Science, Vol. 363, No. 6422, 04.01.2019, p. 81-84.

Research output: Contribution to journalArticle

Xie, KT, Wang, G, Thompson, AC, Wucherpfennig, JI, Reimchen, TE, MacColl, ADC, Schluter, D, Bell, MA, Vasquez, KM & Kingsley, DM 2019, 'DNA fragility in the parallel evolution of pelvic reduction in stickleback fish', Science, vol. 363, no. 6422, pp. 81-84. https://doi.org/10.1126/science.aan1425
Xie KT, Wang G, Thompson AC, Wucherpfennig JI, Reimchen TE, MacColl ADC et al. DNA fragility in the parallel evolution of pelvic reduction in stickleback fish. Science. 2019 Jan 4;363(6422):81-84. https://doi.org/10.1126/science.aan1425
Xie, Kathleen T. ; Wang, Guliang ; Thompson, Abbey C. ; Wucherpfennig, Julia I. ; Reimchen, Thomas E. ; MacColl, Andrew D.C. ; Schluter, Dolph ; Bell, Michael A. ; Vasquez, Karen M. ; Kingsley, David M. / DNA fragility in the parallel evolution of pelvic reduction in stickleback fish. In: Science. 2019 ; Vol. 363, No. 6422. pp. 81-84.
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