Validation of luminescent source reconstruction using spectrally resolved bioluminescence images

Jack Virostko, Alvin C. Powers, E. D. Jansen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This study examines the accuracy of the Living Image® Software 3D Analysis Package (Xenogen, Alameda, CA) in reconstruction of light source depth and intensity. Constant intensity light sources were placed in an optically homogeneous medium (chicken breast). Spectrally filtered images were taken at 560, 580, 600, 620, 640, and 660 nanometers. The Living Image® Software 3D Analysis Package was employed to reconstruct source depth and intensity using these spectrally filtered images. For sources shallower than the mean free path of light there was proportionally higher inaccuracy in reconstruction. For sources deeper than the mean free path, the average error in depth and intensity reconstruction was less than 4% and 12%, respectively. The ability to distinguish multiple sources decreased with increasing source depth and typically required a spatial separation of twice the depth. The constant intensity light sources were also implanted in mice to examine the effect of optical inhomogeneity. The reconstruction accuracy suffered in inhomogeneous tissue with accuracy influenced by the choice of optical properties used in reconstruction.

Original languageEnglish (US)
Title of host publicationDesign and Quality for Biomedical Technologies
PublisherSPIE
ISBN (Print)9780819470249
DOIs
StatePublished - Jan 1 2008
EventDesign and Quality for Biomedical Technologies - San Jose, CA, United States
Duration: Jan 21 2008Jan 21 2008

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6849
ISSN (Print)1605-7422

Other

OtherDesign and Quality for Biomedical Technologies
CountryUnited States
CitySan Jose, CA
Period1/21/081/21/08

Fingerprint

Bioluminescence
bioluminescence
Light sources
Light
light sources
Software
mean free path
computer programs
Optical properties
chickens
Tissue
Chickens
Breast
breast
mice
inhomogeneity
optical properties

Keywords

  • BLI
  • Bioluminescence imaging
  • Bioluminescence reconstruction
  • Bioluminescent tomography
  • Diffusion approximation
  • Validation

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Virostko, J., Powers, A. C., & Jansen, E. D. (2008). Validation of luminescent source reconstruction using spectrally resolved bioluminescence images. In Design and Quality for Biomedical Technologies [68490I] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6849). SPIE. https://doi.org/10.1117/12.771416

Validation of luminescent source reconstruction using spectrally resolved bioluminescence images. / Virostko, Jack; Powers, Alvin C.; Jansen, E. D.

Design and Quality for Biomedical Technologies. SPIE, 2008. 68490I (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6849).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Virostko, J, Powers, AC & Jansen, ED 2008, Validation of luminescent source reconstruction using spectrally resolved bioluminescence images. in Design and Quality for Biomedical Technologies., 68490I, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 6849, SPIE, Design and Quality for Biomedical Technologies, San Jose, CA, United States, 1/21/08. https://doi.org/10.1117/12.771416
Virostko J, Powers AC, Jansen ED. Validation of luminescent source reconstruction using spectrally resolved bioluminescence images. In Design and Quality for Biomedical Technologies. SPIE. 2008. 68490I. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.771416
Virostko, Jack ; Powers, Alvin C. ; Jansen, E. D. / Validation of luminescent source reconstruction using spectrally resolved bioluminescence images. Design and Quality for Biomedical Technologies. SPIE, 2008. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
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